What does an ELISA diagram show?

It shows how the assay is built in a well: what is coated, what binds next, and where the enzyme sits. Teaching figures usually start with a sandwich ELISA. This generator draws that set as SVG.

Is this the same as a western blot?

No. A western blot separates proteins on a gel and transfers them to a membrane. ELISA happens in a coated well. If you need lanes and a ladder, use the gel electrophoresis diagram generator.

What is the difference between sandwich, direct, and indirect ELISA?

Sandwich uses a capture antibody under the antigen. Direct coats antigen and uses one enzyme-conjugated antibody. Indirect coats antigen, then a primary antibody, then an enzyme-conjugated secondary. Switch to Four types to see all four on one figure.

Can I download a blank ELISA worksheet?

Yes. Choose Blank worksheet on the Precise Diagram tab and download the SVG. The well and layer positions stay in the same places as the labeled sandwich.

Does this plot real plate-reader data?

No. The stacks are hardcoded teaching cartoons. For a quantification curve, use the dose-response curve generator. This page is the method figure.

Is the ELISA diagram generator free?

The precise, engine-drawn SVG is free to use and free to download, with no sign-up. Sign in only if you want AI illustrations or other ConceptViz tools. AI file downloads on this page use the journal takeaway wall.

Biology Tool

ELISA Diagram Generator sandwich method figure

Free ELISA diagram generator. Draw a labeled sandwich, direct, indirect, or competitive method figure as SVG — not a western blot.

Sandwich stack with locked layer orderDirect, indirect, and competitive typesProtocol steps or a 96-well plate mapDownload a watermark-free SVG — free

Method figure as SVG: sandwich, types, competitive, protocol, or plate map — free, no account

Figure options

Layer order is a teaching stack, not a plate-reader export. Types and competitive modes change what sits on the plate.

Title

ELISA Diagram Examples

All six images are exact engine renders of the Precise Diagram tab.

View:

Labeled sandwich

Exact engine render — plate, capture, antigen, detection, enzyme.

labeledsandwich

Blank worksheet

Exact engine render — same stack, names removed for revision.

blankunlabeled

Four types

Exact engine render — only sandwich keeps a capture antibody under the antigen.

directindirecttypes

Competitive

Exact engine render — more unlabeled sample means less labeled signal.

competitivelabeled-antigen

Protocol steps

Exact engine render — the sandwich built one addition at a time.

protocolflow

96-well plate

Exact engine render — plate map, not a gel or blot.

plate96-well

What an ELISA diagram is

An ELISA diagram is the method figure for an enzyme-linked immunosorbent assay: a well, what is coated on the plate, the antigen, and the antibody–enzyme stack that makes color. This page draws that lecture cartoon as an exact SVG. It is not a western blot and it is not an enzyme lock-and-key worksheet.

What the stacks lock

Sandwich default: plate → capture antibody → antigen → detection antibody → enzyme. Capture sits under the antigen. The enzyme rides the detection antibody, not the capture antibody.
Direct ELISA coats antigen on the plate. There is no capture antibody.
Indirect ELISA adds a primary antibody, then an enzyme-conjugated secondary. The enzyme does not bind the antigen.
Competitive ELISA: sample antigen and labeled antigen compete for the same capture antibody. More unlabeled sample means less color.

Labeled, blank, types, competitive, protocol, plate

Start with the labeled sandwich so the room can see the layer order. Use the blank copy for a self-test. Four types puts direct, indirect, sandwich, and competitive on one axis. Competitive isolates the competition cartoon. Protocol steps number coat, block, sample, detect, and substrate. The plate map is a top-down 96-well grid, not a gel.

Precise SVG vs AI illustration

The Precise Diagram tab is the version for class and papers: layer order is fixed in code. The AI Illustration tab is only for slide mockups. Check every capture and enzyme against the precise stacks before you project the file. Do not ask the AI tab for a western blot.